Figure 4.

Dose-Dependent Effects of GRF4 and GRF10 on AtPIP2;1 Activity in Xenopus Oocytes.

Dose-dependent effects of GRF4 (left) and GRF10 (right) on normalized P_f of oocytes expressing dSA (gray dots) or dSD (black dots) forms of GFP-PIP2;1. Oocytes were co-injected with the indicated quantity of GRF cRNAs and 2.5 ng of dSA or dSD GFP-PIP2;1 cRNA. The higher normalized P_f seen in dSD compared with dSA oocytes in the absence of GRF4 is probably because of self-activation of dSD or its activation by an endogenous oocyte 14-3-3 protein in some oocyte batches. Representative data from one batch of oocytes are shown, with each point representing mean ± se from 10 cells. The effect of genotype was investigated by ANOVA with a dynamic linear model (**P < 0.01; ***P < 0.001).