Figure 5.

Effects of Peptide Co-Injection on the Activation of GFP-PIP2;1-induced Water Transport by GRF4 and GRF10.

Oocytes were injected with 2.5 ng cRNA of dSA or dSD GFP-PIP2;1 in the absence (H₂O; white bars) or presence of 2.5 ng GRF4 [(A); black bars] or GRF10 [(B); black bars] cRNA. After 2 d, the oocytes were re-injected with the indicated peptide (Pep-8 or _pPep-8) or H₂O as a negative control and incubated for 2 h before the water transport assays. Data are means + se from the indicated number of oocytes and 2 independent batches. Different letters indicate statistically different values at P < 0.05, as determined by ANOVA with a generalized linear model followed by a Tukey test.