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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Breast Cancer Res Treat. 2018 Dec 17;174(2):401–412. doi: 10.1007/s10549-018-05087-7

Figure 5: ER S118P does not exhibit increased metastatic or tumorigenic potential.

Figure 5:

A) MCF7 and targeted cell line derivatives were seeded in 6 well plates with and without 1 nM E2. Once confluent, wells were scratched with a pipet tip and monitored over 16 hours for wound closure. Percent healing of the wound by area was determined by subtracting cell coverage at 0 hours from cell coverage at 16 hours. B) Representative images of scratch wound healing. Data are representative of four independent experiments. C) MCF7 and MERSP1 xenograft formation is estrogen-dependent. MCF7 and MERSP1 cells were injected into nude mice with and without estrogen supplementation implants and mice were monitored for 45 days. Both cell lines formed tumors only in the presence of estrogen. Data are shown as mean + SD of five mice per group.