KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat monoclonal antibody CD16/CD32 (Clone 93) | Thermo Fisher | Cat#14–0161-85; RRID:AB_467134 |
| Rat monoclonal antibody TER-119 (Clone TER-119) | Thermo Fisher | Cat#48–5921-82; RRID:AB_1518808 |
| Rat monoclonal antibody CD44 (Clone IM7) | Thermo Fisher | Cat# 12–0441-81; RRID:AB_465663 |
| Syrian hamster monoclonal antibody CD3e (Clone eBio500A2) | Thermo Fisher | Cat# 48–0033-82; RRID:AB_2016704 |
| Armenian hamster monoclonal antibody CD3e (Clone 145–2C11) | Thermo Fisher | Cat# 17–0031-82; RRID:AB_469315 |
| Rat monoclonal antibody CD19 (Clone eBio1D3) | Thermo Fisher | Cat# 17–0193-82; RRID:AB_1659676 |
| Mouse monoclonal antibody NK1.1 (Clone PK136) | Thermo Fisher | Cat# 25–5941-82; RRID:AB_469665 |
| Mouse monoclonal antibody NK1.1 (Clone PK136) | Thermo Fisher | Cat#11–5941-82; RRID:AB_465318 |
| Mouse monoclonal antibody NK1.1 (Clone PK136) | Thermo Fisher | Cat# 17–5941-82; RRID:AB_469479 |
| Rat monoclonal antibody CD335 (NKp46) (Clone 29A1.4) | Thermo Fisher | Cat# 12–3351-82; RRID:AB_1210743 |
| Rat monoclonal antibody CD11b (Clone M1/70) | Thermo Fisher | Cat# 45–0112-82; RRID:AB_953558 |
| Rat monoclonal antibody F4/80 (Clone BM8) | Thermo Fisher | Cat# 11–4801-82; RRID:AB_2637191 |
| Rat monoclonal antibody Ly-6G (Clone 1A8-Ly6g) | Thermo Fisher | Cat# 12–9668-82; RRID:AB_2572720 |
| Rat monoclonal antibody Ly-6C (Clone HK1.4) | Thermo Fisher | Cat# 48–5932-82; RRID:AB_10805519 |
| Rat monoclonal antibody Granzyme B (Clone NGZB) | Thermo Fisher | Cat# 50–8898-80; RRID:AB_11220478 |
| Rabbit Cleaved Caspase-3 (Asp175) (Clone 5A1E) | Cell Signaling Technology | Cat#9664S |
| Mouse m123/IE1 (Clone CROMA101) | Capri | Cat#HR-MCMV-08 |
| 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) | Sigma Aldrich | Cat#10236276001 |
| NK 1.1 (PK136) | InVivoMab | Cat# BE0036; RRID:AB_1107737 |
| Bacterial and Virus Strains | ||
| SG-MCMV (Smith strain) | American Type Culture Collection | ATCC® VR194 |
| Δm157-MCMV | Bubić et al., 2004 | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Brefeldin A Solution (1000X) | Thermo Fisher | Cat# 00–4506-51 |
| Fixation/Permeabilization Solution Kit | BD Bioscience | Cat#554714 |
| CpG-ODN 1826 | Invivogen | Cat#tlrl-1826–5 |
| Foxp3 Staining Buffer Set Kit | Thermo Fisher | Cat#00–5523-00 |
| Red Blood Cell Lysing Buffer Hybri-Max | Sigma Aldrich | Cat#R7757–100ml |
| Methylcellulose-based medium with recombinant cytokines | Stem Cell Technologies | Cat#M3434 |
| (including EPO) for mouse cells | ||
| Fixable Viability Dye eFluor 780 | Thermo Fisher | Cat# 65–0865-18 |
| Intracellular Fixation & Permeabilization Buffer Set | Thermo Fisher | Cat#88–8824-00 |
| iScript cDNA Synthesis Kit | Bio Rad | Cat# 1708891 |
| HOT FIREPol DNA Polymerase | Solis BioDyne | Cat#01–08-01000 |
| Critical Commercial Assays | ||
| FITC Mouse Anti-Ki-67 Set | BD Bioscience | Cat#556026 |
| Annexin V Apoptosis Detection Set PE-Cyanine7 | Thermo Fisher | Cat#88–8130-72 |
| Experimental Models: Cell Lines | ||
| Mouse embryonic fibroblasts (MEFs) | N/A | N/A |
| Experimental Models: Organisms/Strains | ||
| WT (C57BL/6N) | The Jackson Laboratory | C57BL/6N |
| Stat1ΔM/PMN | Wallner et al., 2012 | N/A |
| Stat1fl/fl | Wallner et al., 2012 | N/A |
| Ifnar1fl/fl | Kamphuis et al., 2006 | N/A |
| Ifngr2fl/fl | Lee et al., 2015 | N/A |
| Stat1+/+ Cre (LysMCre cre/+) | Clausen et al., 1999 | N/A |
| Stat1−/− | Durbin et al., 1996 | N/A |
| Il27−/− (Wsx1−/−) | The Jackson Laboratory | B6N.129P2-Il27ratm1Mak/J |
| Oligonucleotides | ||
| m54-fwd-5′-CATCCGTTGCATCTCGTTG-3′, | Sigma Aldrich | N/A |
| m54 rev-5′-ACGTACATCGCTCTCTGCTG-3′ | Sigma Aldrich | N/A |
| Ube2d2-fwd 5′-AGG TCC TGT TGG AGA TGA TAT GTT-3′ | Sigma Aldrich | N/A |
| Ube2d2-rev 5′ -TTGGGAAATGAATTG TCA AGA AA-3′ | Sigma Aldrich | N/A |
| Ube2d2-probe 5′-CCA AAT GAC AGC CCC TAT CAG GGT GG-3′ | Sigma Aldrich | N/A |
| Ccl2 | QIAGEN | Cat# QT00167832 |
| Ccl5 | QIAGEN | Cat# QT01747165 |
| Software and Algorithms | ||
| R Studio | https://www.rstudio.com/ | N/A |