Fig 2. Unilateral lung injury increases injury while improving survival.

A) H&E staining of lung sections taken from C57BL/6 mice 24 hours after undergoing unilateral (injured area shown) or bilateral lung injury demonstrates significantly increased accumulation of cellular infiltration and protein-rich edema fluid when compared to the lung that underwent bilateral (intratracheal) lung injury. B) We subjected mice to right unilateral (n = 7) and bilateral (n = 8) lung injury. Mice were evaluated on an hourly basis and were euthanized if a blinded observer determined that the mouse was obtunded. Differences in mortality were significant, p = 0.0055, when compared using a log-rank (Mantel-Cox) test.