Table 1. Primer sequences used in the study.
S.No | Group | Name of primers | Primer sequence (5’-3’) |
---|---|---|---|
1. | Anaerobes | Bacteroides | F- GGGGTTCTGAGAGGAAG R- ACCCCCCATTGTACCAC |
2. | Peptostreptococcus | F- AACTCCGGTGGTATCAGATG R-GGGGCTTCTGAGTCAGGTA |
|
3. | Peptococcus | F- GGTGCCGCAGTAAACACAATAAGT R- AAGGCCCGGGAACGTATTCA |
|
4. | Clostridium | F- CTCAACTTGGGTGCTGCATTT R- ATTGTAGTACGTGTGTAGCCC |
|
5. | Fusobacterium | F- GAGAGAGCTTTGCGTCC R- TGGGCGCTGAGGTTCGAC |
|
6. | Prevotella | F- CACRGTAAACGATGGATGCC R- GGTCGGGTTGCAGACC |
|
7. | tRNA-linked polymorphic STR loci | AL3 AL5 |
GGATCGATACCCCTCATCTCCA CGCATCTTGCGATAGCCGAG |
8. | D-A5 D-A3 |
CTGGTTAGTATCTTCGCCTGT GCTACACCCCCATTAACAAT |
|
9. | N-K5 N-K3 |
CGAACGGCTGTTAACCGTTA TTCCTAGCTCAGTCGGTAGA |
|
10. | R-R5 R-R3 |
AGCATCAGCCTTCTAAGCTG CTTCCGACTGAGCTAACAAG |
|
11. | STGA-D5 STGA-D3 |
CTCTGGATGCGTAGGTTCAA GTATCTTCGCCTGTCACGTG |
|
12. | S-Q5 S-Q3 |
GTGGTCTAAGGCGTGTGACT GAGATTCTGGTTCTTAGGACCC |