. 2019 Mar 22;13(3):e0007175. doi: 10.1371/journal.pntd.0007175

Table 2. Sequence of primer pairs and cycling parameters employed in PCR analysis.

	CYTOB	TBR	TCS	TVW
FORWARD PRIMER	GACCTCCCAGCTCCATCAAACATCTCATCTTGATGAAA	GAATATTAAACAATGCGCAG	CGAGAACGGGCACTTTGCGA	CTGAGTGCTCCATGTGCCAC
REVERSE PRIMER	CTCAGATTCACTCGACGAGGGTAGTA	CCATTTATTAGCTTTGTTGC	GGACAAACAAATCCCGCACA	CCACCAGAACACCAACCTGA
ANNEALING TEMPERATURE	60°C	55°C	60°C	62°C
NUMBER OF CYCLES	32	35	35	30^a/35^b
AMPLICON SIZE	439bp	164bp	315bp	150bp

CytoB, cytochrome B; TBr, T. brucei; TCS, T. congolense Savannah; TVW, T. vivax West.

^aCycle number employed for analysis of DNA extracted from whole blood.

^bCycle number used following DNA extraction from FTA card.