Table 2.
Treatment12 | P-value | |||||||
---|---|---|---|---|---|---|---|---|
Item | EN-S | EN-W | EI-S | EI-W | SEM | Fescue | Genotype | Interaction |
Follicle number3 | ||||||||
Preselected follicles4 | 12.18a | 10.60b | 11.07ab | 13.77c | 0.48 | 0.034* | 0.257 | <0.0001* |
Selected follicles5 | 3.09 | 3.30 | 3.05 | 2.96 | 0.13 | 0.146 | 0.663 | 0.273 |
Preovulatory follicles6 | 0.94a | 0.88a | 0.85a | 0.52b | 0.04 | <0.0001* | <0.0001* | 0.002* |
Ovarian structure sizes | ||||||||
Ovulatory follicle, mm | 9.88ab | 11.57a | 10.25ab | 9.14b | 0.69 | 0.150 | 0.674 | 0.053† |
Luteal area, mm2 | 297.2a | 272.7a | 276.8a | 235.1b | 11.7 | 0.015* | 0.005* | 0.467 |
Reproductive hormones | ||||||||
Estradiol, pg/mL | 6.57a | 4.97b | 7.78a | 4.58b | 1.82 | 0.447 | 0.0001* | 0.191 |
Progesterone, ng/mL | 2.38ab | 3.01a | 1.97ab | 1.53b | 0.46 | 0.035* | 0.837 | 0.243 |
Prolactin, ng/mL | 140.9ab | 186.3a | 124.9b | 137.2ab | 31.2 | 0.050* | 0.057† | 0.873 |
PSPB7, ng/mL | 3.36 | 3.05 | 3.13 | N/A | 0.28 | 0.515 | 0.430 | 0.848 |
Within row, means without a common superscript significantly differ (P ≤ 0.05).
Within row, means without a common superscript significantly differ (P ≤ 0.05).
Within row, means without a common superscript significantly differ (P ≤ 0.05).
Values are reported as least square means for the experiment.
EN-S = novel endophyte fescue with slick hair coat; EN-W = novel endophyte fescue with wildtype hair coat; EI-S = endophyte-infected fescue and slick hair coat; EI-W = endophyte-infected fescue with wildtype hair coat.
Follicle number is defined as a follicle observed during ovary mapping via transrectal ultrasonography.
Follicles were classified based on size: preselected follicles 2 to 4 mm, selected follicles 5 to 8 mm, and preovulatory follicles ≥9 mm.
Follicles were classified based on size: preselected follicles 2 to 4 mm, selected follicles 5 to 8 mm, and preovulatory follicles ≥9 mm.
Follicles were classified based on size: preselected follicles 2 to 4 mm, selected follicles 5 to 8 mm, and preovulatory follicles ≥9 mm.
PSPB = pregnancy-specific protein B.
P-values < 0.05 determined significant.
P-values 0.05 > P ≤ 0.10 determined a statistical tendency.