Table 2.
Sequence of primers for real-time PCR
| Target gene1 | Accession no. | Primer sequence (5′ to 3′)2 | Product size, bp |
|---|---|---|---|
| Nrf2 | RA_011763 | F: GAAAGCCCAGTCTTCATTGC | 190 |
| R: TTGGAACCGTGCTAGTCTCA | |||
| GPX1 | NC_010444.3 | F: GGCACAACGGTGCGGGACTA | 159 |
| R: AGGCGAAGAGCGGGTGAGCA | |||
| Keap1 | NM_001114671.1 | F: GCCTCATCGAGTTCGCTTAC | 105 |
| R: CACGGACCACACTGTCAATC | |||
| HO1 | NM_001004027.1 | F: GCTGAGAATGCCGAGTTCAT | 142 |
| R: TGTAGACCGGGTTCTCCTTG | |||
| NQO1 | NM_ 001159613.1 | F: TGAATTACATCTCTGTGGTTTA | 171 |
| R: AGAATGACACTCATATTAGGCG | |||
| GCLM | CV868255.1 | F: ACAATACAACGGTTCAGGTGAGT | 122 |
| R: GCCTGTAAAATGTGTCATTGAGG | |||
| GAPDH | NM_001206359.1 | F: ATGGTGAAGGTCGGAGTGAA | 154 |
| R: CGTGGGTGGAATCATACTGG |
1 Nrf2 = nuclear factor erythroid 2–related factor 2; Keap1 = Kelch-like erythroid cell-derived protein with CNC homology (ECH)–associated protein 1; GPX1 = glutathione peroxidase 1; HO1 = hemeoxygenase 1; NQO1 = quinone oxidoreductase 1; GCLM = modifier subunit of glutamate-cysteine ligase; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.
2F = forward primer; R = reverse primer.