Table 2.
Analytical details regarding quantification of bacterial gene expression
| Target | Gene | Primer sequences1 | Amplicon | Reference |
|---|---|---|---|---|
| F18+E. coli | fedA | 5′-GTGAAAAGACTAGTGTTTATTTC-3′ | 510 bp | DebRoy and Maddox, 2001 |
| 5′-CTTGTAAGTAACCGCGTAAGC-3′ | ||||
| Total E. coli | gadAB | 5′-GCGTTGCGTAAATATGGTTGCCGA-3′ | 305 bp | Chen et al., 2006 |
| 5′-CGTCACAGGCTTCAATCATGCGTT-3′ | ||||
| Total bacteria | rrs | 5′-GGATTAGATACCCTGGTAGTCC-3′ | 320 bp | Kariyama et al., 2000 |
| 5′-TCGTTGCGGGACTTAACCCAAC-3′ |
1Forward and reverse sequences are shown on the top and bottom line, respectively, for each gene.