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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Biomaterials. 2019 Feb 23;202:26–34. doi: 10.1016/j.biomaterials.2019.02.021

Figure 3. Type and concentration of biochemical cues alter stiffness-dependent cytoskeletal organization and YAP regulation in hPSCs.

Figure 3.

a Effects of the type of biochemical cue on hPSC mechanotransduction. rhLaminin- and GAG-derived peptides-conjugated hydrogels rescued stiffness-dependent YAP localization. White arrowhead indicates nucleus that is negative for YAP expression. Scale bar: 20 μm. b Concentration-dependent effect of Matrigel on hPSC mechanosensing of substrate stiffness. Mechanosensing of bulk hydrogel stiffness appeared only with an intermediate amount of Matrigel coating (1:400). In contrast, high Matrigel conjugation (1:40) led hPSCs to sense a ‘stiff’ environment and low Matrigel conjugation (1:1000) led to sensing of a ‘soft’ microenvironment regardless of ‘bulk’ hydrogel stiffness. Scale bar: 20 μm. c,d Quantification of nuclear YAP localization. Data are mean values; error bars are standard error of the mean. (n=14, **p<0.01, ****p<0.0001, two-way ANOVA by Bonferroni’s multiple comparisons test)