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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Biomaterials. 2019 Feb 23;202:26–34. doi: 10.1016/j.biomaterials.2019.02.021

Figure 4. Concentration of fibronectin alters stiffness-dependent cytoskeletal organization and YAP regulation in hMSCs.

Figure 4.

a Concentration-dependent effect of fibronectin on hMSC sensing of substrate stiffness. While high fibronectin coating (10 μg/ml) led hMSCs to sense a ‘stiff’ environment, low fibronectin coating (1 μg/ml) led to sensing of a ‘soft’ microenvironment regardless of hydrogel stiffness. In the groups with 4 or 7 μg/ml fibronectin, YAP regulation was dependent on substrate stiffness, as shown in the conventional polyacrylamide hydrogel groups. Scale bar: 20 μm. b Quantification of nuclear YAP localization. Data are mean values; error bars are standard error of the mean. (n=14, ****p<0.0001, two-way ANOVA by Bonferroni’s multiple comparisons test. n.s. not significant)