Table 1.

Gene names, Ovis aires accession numbers (National Center for Biotechnology Information), and forward and reverse primer sequences for genes assessed with real-time PCR

Gene1	Symbol	Accession number	Primer sequence 5′ → 3′ (forward; reverse)	Amplicon (bp)
β-Actin	ACTB	NM_001009784.1	TCC ACC GCA AAT GCT TCT CAC GAG GCC AAT CTC ATC TC	99
Glyceraldehyde 3-phosphate dehydrogenase	GAPDH	NM_001190390.1	AGT TCC ACG GCA CAG TCA AG AGG ATC TCG CTC CTG GAA GAT	83
Ribosomal protein, larger, P0	RPLP0	XM_004017413.2	CTC TGG AGA AAC TGT TGC CT TAT TGG CCA GCA GCA TGT	96
α-Adrenergic receptor 1A	ADRA1A	XM_015093082.1	GCC CAT TGG GTC TTT CTT TC GGG TTG ATG CAG CTG TTT AG	93
5-Hydroxytryptamine receptor 1B	HTR1B	XM_015097299.1	CCA CGG AAT TGA CTG AGA TAG G GGG AAG GAG AGT GCA AAT GA	138
5-Hydroxytryptamine receptor 1D	HTR1D	XM_015093689.1	GGG AGA GGA AAG CCA CAA A GGA CCA GAG ATG CTA CAA AGA A	87
5-Hydroxytryptamine receptor 2A	HTR2A	XM_004012066.3	GCA GAA TGC CAC CAA CTA TTT C ACC GGT ACC CAT AGA GGA TG	107
Guanine nucleotide-binding protein G(s) subunit alpha	GNAS	XM_012188911.1	CGC ACC ATC TCT GTG ATT CT GCG AGC AAA TTC TGG AAA GTA G	105
G protein subunit alpha q	GNAQ	XM_015093177.1	GTA CGA GCA CAA TAA GGC TCA CTC GTC GTC TGT CAT AGC ATT C	143
G protein subunit alpha 11	GNA11	XM_015095900.1	GTG GAG TCA GAC AAC GAG AA GAG GAA GAG GAT GAC AGA TGA G	102
Nitric oxide synthase 3	NOS3	NM_001129901.1	TCC CTG TAC TAT CTC ATC CTC TC CTG ACT GTA GGC TCT GGA ATA C	101

¹ β-Actin, GAPDH, and RPLP0 are reference genes used to normalize gene expression of target genes.