Table 5. Top 10 lipid and gene expression markers associated with notch signaling (Notch), epithelial mesenchymal transition (EMT) and cancer1.
| Pathway | Notch | EMT | Cancer | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Marker | Hey1 | S100A4 | Gpc3 | ||||||
| Association | Feature | CC2 | FDR3 | Feature | CC | FDR | Feature | CC | FDR |
| Positive | CtsS | 0.97 | 6.4E-13 | Mpk | 0.96 | 1.1E-10 | CtsS | 0.83 | 9.1E-05 |
| Positive | HeyL | 0.97 | 2.1E-12 | MIS | 0.95 | 5.5E-10 | HeyL | 0.82 | 9.1E-05 |
| Positive | CD68 | 0.96 | 2.3E-11 | TG 51:1 | 0.92 | 2.3E-08 | Sox4 | 0.81 | 9.1E-05 |
| Positive | Tgfβ1 | 0.95 | 4.2E-10 | DG 34:1 | 0.92 | 2.4E-08 | CD40 | 0.81 | 9.1E-05 |
| Positive | CD44 | 0.95 | 4.8E-10 | 18:1,ω9 | 0.92 | 2.4E-08 | Hey1 | 0.81 | 9.1E-05 |
| Positive | MIS4 | 0.95 | 5.1E-10 | Hey1 | 0.91 | 2.8E-08 | Cox2 | 0.81 | 9.1E-05 |
| Positive | Bcl2 | 0.94 | 2.3E-09 | MUFA | 0.91 | 2.8E-08 | Hmox1 | 0.81 | 9.1E-05 |
| Positive | Casp1 | 0.93 | 3.8E-09 | 14:0 | 0.91 | 2.8E-08 | CD68 | 0.80 | 9.1E-05 |
| Positive | F4/80 | 0.92 | 1.0E-08 | HeyL | 0.91 | 3.3E-08 | Tgfβ1 | 0.80 | 1.1E-04 |
| Positive | Cxcl14 | 0.92 | 1.0E-08 | DG 36:1 | 0.91 | 4.5E-08 | Cxcl14 | 0.79 | 1.1E-04 |
| Negative | GpCho 36:5 | -0.92 | 1.1E-08 | ω3 index | -0.94 | 9.7E-10 | 8,9-EpETE | -0.82 | 9.1E-05 |
| Negative | ω6 Index | -0.92 | 1.1E-08 | ω6 Index | -0.94 | 9.7E-10 | GpEtn 42:8 | -0.81 | 9.1E-05 |
| Negative | ω3 index | -0.92 | 1.1E-08 | GpCho 36:5 | -0.92 | 2.0E-08 | GpIns 36:2 | -0.81 | 9.1E-05 |
| Negative | GpSer 40:6 | -0.90 | 5.9E-08 | TG 58:9 | -0.92 | 2.4E-08 | GpCho 36:4 | -0.80 | 9.1E-05 |
| Negative | TG 58:9 | -0.90 | 6.8E-08 | GpSer 40:6 | -0.91 | 3.3E-08 | TG 62:13 | -0.80 | 1.1E-04 |
| Negative | Total GpSer | -0.90 | 8.0E-08 | Total GpSer | -0.90 | 6.3E-08 | Tgh | -0.79 | 1.1E-04 |
| Negative | Pdk4 | -0.89 | 9.4E-08 | GpCho 38:4 | -0.89 | 1.4E-07 | TG 58:9 | -0.79 | 1.1E-04 |
| Negative | 11,12-EpETE | -0.89 | 1.2E-07 | GpEtn 38:6 | -0.88 | 2.1E-07 | GpIns 40:6 | -0.79 | 1.1E-04 |
| Negative | GpEtn 36:5 | -0.88 | 2.3E-07 | GpEtn 36:2 | -0.88 | 2.7E-07 | GpCho 36:5 | -0.78 | 1.5E-04 |
| Negative | GpEtn 36:2 | -0.88 | 2.8E-07 | 11,12-EpETE | -0.88 | 2.9E-07 | GpCho 40:8 | -0.78 | 1.5E-04 |
1Associations between NASH features were determined using the statistical package "Pattern Hunter" in Metabolanalyst [37, 38, 53]. The markers chosen for this analysis were based on the most robust response to the WD within each pathway.
2Correlation coefficient;
3False discovery rate;
4MIS, microsteatosis