Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (HEK293T) | HEK293T | ATCC | Tested negative for mycoplasma. | |
| Antibody | Rabbit anti-HA | Cell Signaling Technology | C29F4 | 1:100 |
| Antibody | Goat anti-Rabbit AlexaFluor 647 | Life Technologies | A27040 | 1:1000 |
| Antibody | Mouse anti-Flag | Sigma | F3165 | 1:300 |
| Antibody | Goat anti-Mouse AlexaFluor 568 | Life Technologies | A11004 | 1:1000 |
| Recombinant DNA reagent |
See Table 1 for all plasmids used or generated in this study. | |||
| Commercial assay or kit |
Nano-Glo Live Cell Assay System | Promega | 1:100 substrate | |
| Commercial assay or kit |
Bright-Glo Luciferase Assay System | Promega | 1:1 substrate | |
| Software, algorithm |
Fiji | Schindelin et al., 2012 | ||
| Software, algorithm |
CellSegm toolbox | Hodneland et al., 2013 | ||
| Software, algorithm |
MATLAB R2017a | Mathworks |