Figure 6 – Pharmacological evidence for Ih in human supragranular pyramidal neurons.
A) Bath application of 10 μM ZD7288 hyperpolarized human neurons by ~8 mV (p < 0.001, Bonferroni’s post hoc comparison), but no consistent change in mouse neurons was observed (p = 0.56, Bonferroni’s post hoc comparison). The plot at the left shows RMP as a function of time for two example recordings. B) 10 μM ZD7288 produced an increase in input resistance in human and mouse supragranular pyramidal neurons (p < 0.001, MixedANOVA effect of ZD7288). The percent change in input resistance was larger in human compared with mouse (p < 0.001, t-test). Example voltage responses to hyperpolarizing current injections are shown to the left. C) 10 μM ZD7288 increased the excitability of human (p < 0.001, RM ANOVA), but not mouse pyramidal neurons (p = 0.13, RM ANOVA). Plots are averages from 11 human neurons from 4 patients and 10 mouse neurons from 6 animals. Data are presented as mean ± SEM.