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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Proteomics. 2019 Feb 20;19(5):e1800389. doi: 10.1002/pmic.201800389

Figure 1. Experimental workflow.

Figure 1.

The experimental strategy entailed eight stages. 1. Mouse pathogen administration. 2. Sepsis monitoring and control of bacterial load in pre-septic and septic mice. 3. Blood collection by cardiac puncture for bacterial load determination and proteomics studies. 4. Platelet-poor plasma preparation from blood. 5. Sample processing for proteomics analysis. 6. Proteomics analysis by 2D-LC (high/low pH) and high-resolution MS/MS. 7. Protein abundance determination by LFQ in SPN and ST; and orthogonal validation of protein abundances by TMT in EC, MRSA, SA, SPN and ST. 8. Protein network integration from functional annotation databases and manual curation.