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. 2019 Apr 3;5(4):eaau6547. doi: 10.1126/sciadv.aau6547

Fig. 1. MMP-10 is increased in gastric mucosa of H. pylori–infected patients and mice.

Fig. 1

(A) Representative MMP array results for human primary gastric mucosa of H. pylori–infected patients and paired uninfected donors using Ab microarrays. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software (ImageJ, National Institutes of Health). (B) MMP-10 mRNA expression in gastric mucosa of H. pylori–infected (n = 65) and uninfected donors (n = 40) was compared. (C) The correlation between MMP-10 expression and H. pylori colonization in gastric mucosa of H. pylori–infected patients was analyzed. (D) MMP-10 mRNA expression in gastric mucosa of cagA+ H. pylori–infected (n = 34), cagA− H. pylori–infected (n = 31),and uninfected donors (n = 40) was compared. (E) Dynamic changes of MMP-10 mRNA expression in gastric mucosa of WT H. pylori–infected, ΔcagA-infected, and uninfected mice. n = 5 per group per time point in (E). (F and G) MMP-10 protein in gastric mucosa of cagA+ H. pylori–infected, cagA− H. pylori–infected, and uninfected donors or in gastric mucosa of WT H. pylori–infected, ΔcagA-infected, and uninfected mice at 9 weeks p.i. was analyzed by Western blot (F) and immunohistochemical staining (G). Scale bars, 100 μm. (H) MMP-10 mRNA expression and MMP-10 protein in/from human primary gastric mucosa from uninfected donors infected with WT H. pylori or ΔcagA ex vivo analyzed by real-time polymerase chain reaction (PCR), Western blot, or enzyme-linked immunosorbent assay (ELISA) (n = 8). The horizontal bars in (B), (D), and (H) represent mean values. Each ring or dot in (B) to (D) and (H) represents one patient or donor. *P < 0.05, **P < 0.01 for groups connected by horizontal lines or compared with uninfected mice. GAPDH, glyceraldehyde phosphate dehydrogenase.