FIG 2.

Zfp423 expression is downregulated during myogenic differentiation. (A) Primary satellite cells were plated and cultured in adherent culture conditions in growth medium (GM) or differentiation medium (DM) for 3 days, after which qRT-PCR analysis of Zfp423 and Myomaker expression was performed. (B) qRT-PCR of Zfp423 and Myomaker gene expression in undifferentiated proliferating C2C12 cells (day 0) and over the course of 4 days after the induction of myoblast differentiation. (C) qRT-PCR of Zfp423 and Myomaker expression in Sol8 myoblast cells during differentiation for the indicated number of days. (D) qRT-PCR showing Zfp423 and Myomaker expression during the course of muscle regeneration. TA muscles were injected with cardiotoxin (CTX) and harvested on the indicated days after injury for RNA. The data are presented as means ± the SD (n ≥ 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001 (relative to GM or day 0; Student's t test).