FIG 3.
Zfp423 is critical for muscle regeneration in vivo. (A) Schematic of CTX injury model. (B) Expression of Zfp423 in TA muscles of 7-week-old Pax7Cre; Zfp423f/f (Zfp423cko) mice. (C) H&E staining of TA muscles from control and Zfp423cko mice in uninjured conditions (day 0), as well as 7 and 21 days after injury. (D and E) Cross-sectional area (D) and minimal Feret’s diameter (E) of regenerating myofibers at the indicated time points following injury. (F) Ki67 immunostaining and quantification on control and Zfp423cko TA muscles at day 7 after injury. (G and H) Pax7 immunostaining (G) and quantification on control and Zfp423cko TA muscles (H) at days 0, 7, and 21 after injury. The data are presented as means ± the SD (n ≥ 3). n.s, not significant; *, P < 0.05; ***, P < 0.001 (Student's t test). Scale bars: 65 μm (C) and 100 μm (F and G).