Extended Data Fig. 8 |. Social interaction disrupts caloric intake.

a–d, Setup for separate, counterbalanced 10-min sessions of free-access caloric licking during baseline (a), novel object (b), juvenile social interaction (c) and adult social interaction (d). e, Average cumulative lick rate (30-s bins) across 10-min baseline, novel object (3D-printed mouse), juvenile and adult social-interaction sessions (n = 6 mice). f, GCaMP6m + bReaChES mice display decreased cumulative licking within the first 2 min of the juvenile social-interaction session when compared to the first 2 min of the baseline, novel-object and adult social-stimulus sessions. n = 6 mice; interaction F_5,15 = 3.398, P = 0.029, one-way ANOVA with repeated measures. g, Cumulative licking did not significantly differ within the last 2 min of the baseline, novel-object, juvenile and adult social-interaction sessions. n = 6 mice; interaction F_5,15 = 1.714, P = 0.192, one-way ANOVA with repeated measures. h, Time course for interactions with juvenile, adult and novel object during each 2-min bin across each 10-min free-access licking session. n = 6 mice. i, Time spent interacting with juvenile conspecifics was significantly greater during the first 2 min than the last 2 min of the 10-min session and when compared to interactions with adult conspecifics and novel object. n = 6 mice; interaction F_2,10 = 15.53, P = 0.0009, two-way ANOVA with repeated measures. *^#P and **^#P significant across all comparisons; n.s., non-significant (P > 0.05).