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. Author manuscript; available in PMC: 2020 Apr 2.
Published in final edited form as: Structure. 2019 Feb 14;27(4):639–650.e2. doi: 10.1016/j.str.2019.01.009

Figure 1. NMR spectra of KIR3DL1 cytoplasmic region.

Figure 1.

(A) Sequence of SET-3DL1-cyto with basic residues in light blue, acidic residues in red, ITIMs in green, and ITIM tyrosines with red filled squares. SET sequence is under black bar. We divided 3DL1-cyto into three segments: Segment I (red bar; H340-V351), Segment II (blue bar; M352-D371), and Segment III (green bar; P372-P423). Residues denoted by black filled boxes are involved in binding to N-SH2 (from Fig. 7). (B) 1H-15N HSQC spectrum of 1 mM SET-3DL1-cyto recorded at 37°C in 20 mM HEPES (pH 7.4), 20 mM NaCl, 3 mM EDTA, and 3 mM DTT. (C) 13C-detected CON spectrum of 1 mM SET-3DL1-cyto recorded at 37°C in 20 mM HEPES (pH 7.4), 20 mM NaCl, 3 mM EDTA, and 3 mM DTT. Peaks are labeled based on nitrogen chemical shifts. Positions of prolines are likely folded inside the spectrum due to spectral width used in the nitrogen dimension. The 28 peaks assigned to residues in the SET have red numbering, and 70 peaks assigned from 3DL1-cyto are in black.