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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Biotechnol Adv. 2019 Feb 20;37(3):476–490. doi: 10.1016/j.biotechadv.2019.02.013

Fig. 3.

Fig. 3

Digitization. Samples are partitioned into individual PCR reactions so that no single partition contains more than 1 copy of the DNA template. The quantification is accomplished by counting the number of positive partitions. The digitization also applies to cells by partitioning a single cell into each compartment. By digitization, polymicrobial samples are physically deconvoluted to “monomicrobial” samples. There is only one pathogen in each partition, and the combined information from all partitions reveals the composition of the polymicrobial sample.