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. Author manuscript; available in PMC: 2020 Apr 2.
Published in final edited form as: Cell Metab. 2019 Feb 14;29(4):1003–1011.e4. doi: 10.1016/j.cmet.2019.01.014

Figure 2. Serine metabolism supports LPS induction of GSH synthesis, which is required for IL-1β gene expression.

Figure 2.

(A) Total ion counts of NEM derivitized GSH and GSSG in peritoneal macrophages (n=3).

(B) IL-1β and (C) IL-10 mRNA expression in peritoneal macrophages stimulated with 100ng/mL LPS for 4 hours in control or serine-depleted media, supplemented with 1 mM or 5mM cell permeable glutathione (GSH) reduced ethyl ester. Data is normalized to LPS treated macrophages with serine and glycine and without GSH (n=5).

(D) U-[13C]-Glycine labeling of GSH in peritoneal macrophages (n=3) at 4 hours post LPS treatment.

(E) U-[13C]-Glycine labeling of IMP in peritoneal macrophages (n=3) at 4 hours post LPS treatment.

(F) Glycine uptake over time as measured by the intracellular U-[13C]-Glycine fraction in A549 cells, H460 cells, and peritoneal macrophages untreated or stimulated with LPS for 4 hours.

Data are shown as mean ± SD (A, D-F) or ± SEM (B-C). For B-C, p values were calculated using a one-way paired ANOVA compared to LPS stimulated cells. *p<0.05.