(A) Total ion counts of NEM derivitized GSH and GSSG in peritoneal macrophages (n=3).
(B) IL-1β and (C) IL-10 mRNA expression in peritoneal macrophages stimulated with 100ng/mL LPS for 4 hours in control or serine-depleted media, supplemented with 1 mM or 5mM cell permeable glutathione (GSH) reduced ethyl ester. Data is normalized to LPS treated macrophages with serine and glycine and without GSH (n=5).
(D) U-[13C]-Glycine labeling of GSH in peritoneal macrophages (n=3) at 4 hours post LPS treatment.
(E) U-[13C]-Glycine labeling of IMP in peritoneal macrophages (n=3) at 4 hours post LPS treatment.
(F) Glycine uptake over time as measured by the intracellular U-[13C]-Glycine fraction in A549 cells, H460 cells, and peritoneal macrophages untreated or stimulated with LPS for 4 hours.
Data are shown as mean ± SD (A, D-F) or ± SEM (B-C). For B-C, p values were calculated using a one-way paired ANOVA compared to LPS stimulated cells. *p<0.05.