Fig. 3.

TYROBP deficiency alters microglia phenotype. a Representative images of anti-Iba1 DAB immunohistochemistry in 4-month-old MAPT^P301S mice with (top) and without (bottom) TYROBP. Scale bars = 100, 200 and 50 μm, respectively. b Top left panel: quantification of IBA1 immunoreactivity in cortical areas (n = 4 mice per group with an average of 4–5 slices per mouse). Top right panel: average microglia density (cells/mm²; n = 4 mice per group with an average of 4–5 slices per mouse). Bottom panel: diameter of microglial soma in cortex. n = 20 microglia per mouse and area with n = 4 (MAPT^P301S) or n = 5 (MAPT^P301S;Tyrobp^-/-) mice per group. c Representative images of anti-CD68 immunohistochemistry in the hippocampus of the same groups as in Fig. 3a. Scale bar = 100 μm. d RT-qPCR of CD68 mRNA in the prefrontal cortex in the same mice as in Fig. 3a with n = 4 mice per group. e Representative images of immunohistochemistry with anti-Iba1 antibody in WT or Tyrobp-null mice injected with AAV-tau-GFP in the medial entorhinal cortex (see Fig. 2 and Supplementary Figure 3). Scale bar = 200 μm. Error bars represent means ± SEM. Males and females were used for experiments, and results were combined for analysis. Statistical analyses were performed using a Student’s t-test, *p < 0.05; **p < 0.01; ***p < 0.001