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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: J Biomed Mater Res B Appl Biomater. 2018 Oct 3;107(5):1576–1586. doi: 10.1002/jbm.b.34250

FIGURE 5.

FIGURE 5.

CLSM macrophotographs of 7-day A. naeslundii biofilm growth inside dentinal tubules (control), and infected dentin treated for 7 days with tubular 3D antibiotic-free construct (AF-3DC), tubular 3D triple antibiotic-eluting construct (TA-3DC), or TAP solution. CLSM images were collected from inner root canal walls with a mosaic technique allowing a deeper analysis in sequential illumination mode by using 488-nm and 552-nm laser lines. Fluorescent emission was collected in 2 HyD spectral detectors with filter range set up to 500–550 nm and 590–655 nm for green (SYTO9) and red dye (PI), respectively. Inset Table illustrating median percentage of DEAD and LIVE bacterial cells of each group, demonstrating that TA-3DC eliminated almost all live cells, not differing from TAP solution.