Table 1.
Summary of process parameters and performance during the propagation of CR.pIX cells for all analyzed cultivations
| Cultivation | Working volume [mL] | Hollow fiber module | Initial VCD [106 cells/mL] | μmean [1/h] | μmax [1/h] | Actual average CSPR [nL/(cell × day)] | Duration [h]*** |
|---|---|---|---|---|---|---|---|
| MVA-CR19 virus | |||||||
| Perfusion* | 800 | 500 kDa | 0.8 | 0.019 (n = 12, SD = 0.005) | 0.026 | 0.057 (n = 24, SD = 0.006) | 247 |
| Hybrid 1 | 600 | 0.65 μm | 1.0 | 0.021 (n = 12, SD = 0.006) | 0.029 | 0.068 (n = 18, SD = 0.013) | 191 |
| Hybrid 2 | 600 | 0.20 μm | 8.0 | 0.024 (n = 4, SD = 0.005) | 0.031 | 0.057 (n = 7, SD = 0.011) | 50 |
| Influenza A virus | |||||||
| Perfusion | 800 | 500 kDa | 1.0 | 0.017 (n = 18, SD = 0.011) | 0.055 | 0.072 (n = 24, SD = 0.010) | 183 |
| Hybrid | 600 | 0.65 μm | 1.0 | 0.019 (n = 7, SD = 0.004) | 0.025 | 0.119 (n = 20, SD = 0.061)** | 168 |
| Batch | 800 | NA | 0.8 | 0.020 (n = 6, SD = 0.009) | 0.033 | NA | 82 |
*Vazquez-Ramirez et al. (2018)
**Non-constant CSPR: perfusion rates were automatically adjusted to maintain the pH at a set point of 7.2 and not based on the actual viable cell densities
***Time from cells inoculation to infection