Table 2.

Yields for different cultivation strategies during the virus propagation phase

Cultivation	Working volume [mL]a	Hollow fiber module	Harvest volume [mL]	Harvest titerb	tT [day]c	Maximum viable cell number [109]	Y v/cell d	PVe
MVA-CR19 virus
Perfusion*	800	500 kDa	800	3.2 × 109	13.0	66.4	38	1.0 × 1010
FB + daily harvest (F + D)*	60	NA	95	5.3 × 109	10.0	1.9	270	2.6 × 1011
Hybrid 1	900	0.65 μm	900	1.0 × 1010	10.9	21.9	410	1.3 × 1011
Hybrid 2	750	065 μm	750	1.0 × 1010	8.2	21.3	352	2.8 × 1011
Influenza A virus
Hybrid	810	0.65 μm	810	3.80 × 1010	9.5	23.7	1300	5.43 × 1011
Perfusion	800	500 kDa	800	8.05 × 109	9.6	19.0	340	1.81 × 1011
Batch	800	NA	800	5.23 × 109	8.0	3.1	1344	6.53 × 1011

^aFor FB-based processes: maximum working volume

^bIn IU/mL for MVA virus and total virions/mL(estimated from HA measurements) for influenza A virus

^cTotal time from cell inoculation to maximum titer

^dCell-specific virus yield in IU/cell for MVA virus and total virions/cell (from HA) for influenza A virus

^eVolumetric productivity in IU/(L × day) for MVA virus and virions/(L × day) (from HA) for influenza A virus

*Vazquez-Ramirez et al. (2018)