Table 1.

Guidelines for the isolation of human M-MDSC and PMN-MDSC in blood

Variable	Indication
Time	Isolate cells within one hour after blood withdrawal, avoid use of frozen samples
Anticoagulant	Collect peripheral blood in either ethylenediaminetetraacetic acid (EDTA) or sodium citrate containing tubes
Separation reagent	Use commercially available gradient solutions (1.077 g/L)