Fig. 5. Actin binders reduce DNA-PK autophosphorylation.

a Simplified flow chart of NHEJ. b Numbers of p-DNA-PK (T2609) foci were counted and cells with at least 70 foci/nucleus defined as positive cells, scale bars: 5 µm. c−e Duolink assay was performed according to the manufacturer’s suggestions. Antibodies against actin and DNA-PKcs were used for Duolink assay (nuclear area is shown and nuclear outlines depicted in white) (c). d Antibodies against actin and Ku70 were applied after treatment of cells with Doxo. Positive events were counted for each nucleus for at least three independent experiments. b, d unpaired t test, two-tailed, *p < 0.05. NHEJ nonhomologous end joining