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. 2019 Apr 3;9:5561. doi: 10.1038/s41598-019-41466-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of COL2A1 and ACAN in skeletal cells derived from human fetal femurs chondrogenic culture at day 21, including control (cells cultured with no live-imaging), cells cultured over 21 days and live-imaging performed at day 7, and cells cultured over 21 days and live-imaging performed at both days 7 and 21. Normalisation was carried out with respect to ACTB to obtain relative gene expression and the gene expression on day 0 was assigned a value of 1 (as indicated by the dotted line). Average data from 3 independent samples is shown where error bars indicate the observed standard deviation. The Mann-Whitney test was used to calculate the level of significance at *p-value < 0.05.