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. 2019 Apr 3;9:5599. doi: 10.1038/s41598-019-41753-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analytical method validation of the ddPCR assays. Linearity (a–c) and reproducibility (d–f) of the LINE-1 and mt assays were assessed in triplicate using synthetic gBlocks and digested human gDNA as templates. Data are fitted to linear regression models. Theoretical copy number represents the expected number of molecules based upon the known concentration of the synthetic gBlock solution.