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. 2019 Apr 3;9:5599. doi: 10.1038/s41598-019-41753-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of three different stool preservative solutions for host DNA stabilisation at room temperature. (a) Schematic diagram of stool processing for the stool DNA stability experiment and (b) Effectiveness of different stool preservation solutions on endogenous DNA stability. Change in ACN per μl stool DNA extract from baseline (time 0) of LINE-1 (left), mt (ND5) (middle), and 16S (right) DNA are plotted for varying incubation times of stool specimens at room temperature. TEN2- and EDTA-preserved stools were DNA extracted and analyzed in duplicate; the error bars represent standard errors from ddPCR of two replicate extractions for each time point. The OMNI-preserved stool provided enough material only for one extraction per time point and therefore error bars are not provided.