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. 2019 Apr 3;9:5599. doi: 10.1038/s41598-019-41753-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of host DNA from human patient stools using ddPCR. (a) Total DNA recovered from two side-by-side DNA isolations from 69 stool samples from three hospitalised patients (P07, P12, and P13) undergoing allogeneic HCT. Each circle represent DNA recovered from a single DNA extraction. The horizontal lines represent the median and interquartile range. (b,c) Human DNA quantified using human LINE-1 (60-bp amplicon) and mitochondrial (83-bp amplicon in the ND5 gene) assays. Error bars in (c–f) represent range of copy numbers from single ddPCR reactions on the duplicate DNA extraction. For some points, the error bars would be shorter than the height of the symbol. In these cases, the error bars were not drawn.