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. 2019 Apr 3;9:5562. doi: 10.1038/s41598-019-41724-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Resection of DTA-1-containing TDLN reduced antitumor immunity. (a) The attenuation of antitumor effect of DTA-1-treated TDLN resection. DTA-1 Ab was injected into tumors 7 days after tumor inoculation, following TDLN resection 4 hour after Ab injection (n = 6). Tumor volumes were measured at the indicated days. (b) The effect of DTA-1 Ab-injected TDLNs on antitumor effect. CT26 subcutaneous tumors were established on both legs of BALB/c mice. Then, DTA-1 Ab was injected into one side, following TDLN resection of the same side (black circle) or the opposite side (black triangle) (n = 6). P values for volume of DTA-1-injected tumor with removal of TDLNs of injected tumors vs DTA-1-injected tumor with removal of TDLNs of uninjected tumors (*), and volume of DTA-1-uninjected tumor with removal of TDLNs of injected tumors vs DTA-1-uninjected tumor with removal of TDLNs of uninjected tumors (**), as analysed by t-test.