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. 2019 Apr 3;9:5581. doi: 10.1038/s41598-019-41936-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic clustering of LusHSFs, AtHSFs and OsHSFs. The phylogenetic relationship tree was inferred from the Maximum Likelihood (ML) method and JTT + G + I matrix-based model in MEGA-X. Domain-centric alignment of amino acid sequences from DBD and OD domains were performed using the MUSCLE algorithm with maximum 16 iterations. Thirty-four LusHSF, 21 AtHSF and 33 OsHSF proteins were clustered into 3 broad classes A, B, and C and 15 sub-classes within. Sub-groups marked in grey did not consist any LusHSFs. Bootstrap support values of >50% are shown on the nodes.