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. 2019 Apr 3;9:5581. doi: 10.1038/s41598-019-41936-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative quantification (RT-qPCR) of selective LusHSF genes between HT-treated and control plants of JRF-2 and Viking cultivars. (a) The upper panel shows the effect of HT stress treatments at 40 °C for 12 hrs in flax cultivars, which were used for total RNA extractions. (b) The relative gene expression fold change (2^−ΔΔCt) of twelve selected LusHSF genes are represented as bar diagrams. The Ct values of each sample-HSF gene combination were normalized using the reference gene ETIF3E and calibrated with Ct values of Viking control to estimate (2^−ΔΔCt) values. The statistical significance of the expression values is represented by ‘ns’ as non-significant, and ‘*‘ as significant at p > 0.05 and Bonferroni’s multiple comparisons test. One asterisk (*) represents adjusted P values between 0.01 and 0.05, and two asterisks (**) represent adjusted P values between 0.01 and 0.001, and so on. RNA samples include Vik_C: Viking under control conditions; Vik_H: Viking under HT stress conditions; JRF-2_C: JRF-2 under control conditions; JRF-2_H: JRF-2 under HT stress conditions.