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. 2019 Mar 28;6:31. doi: 10.3389/fnut.2019.00031

Figure 3.

Figure 3

Changes of mRNA expression levels of cell cycle genes (cyclin A, B, D, E) in intestinal HT-29, Caco-2 and HIE cells using GAPDH as housekeeper gene. Cells were treated with 10 mg/mL BMO from rHF, bHF, SIM and JER after reaching a confluency of 30 % over 72 h. Then, mRNA expression levels were determined using the target gene/housekeeping gene ratio by setting the control to 100%. Values are means of the percentage of controls with their standard error (n = 3). Mean values were significantly different from those of the control group: *P ≤ 0.05, **P ≤ 0.01, ***P<0.001.