Table 1.
Distribution of cell cycle phases after BMO incubation.
| HT-29 | |||||
|---|---|---|---|---|---|
| Control | rHF | bHF | SIM | JER | |
| sub G0/G1 | 0.4% | 0.5% | 0.3% | 0.5% | 0.4% |
| G0/G1 | 71.3% | 49.3%* | 53.2%* | 56.2%* | 48.3%* |
| S | 10.9% | 10.3% | 10.6% | 11.3% | 10.4% |
| G2/M | 14.5% | 35.5%** | 32.4%* | 30.3%* | 34.3%** |
| Caco-2 | |||||
| sub G0/G1 | 0.7% | 0.5% | 0.6% | 0.6% | 0.4% |
| G0/G1 | 74.9% | 49.5%* | 66.4%* | 65.5%* | 54.4%* |
| S | 5.4% | 9.2% | 11.5% | 12.2% | 10.6% |
| G2/M | 12.2% | 39.3%** | 34.4%* | 31.1%* | 37.5%** |
| HIEC | |||||
| sub G0/G1 | 1.5% | 0.8% | 1.6% | 1.8% | 0.7% |
| G0/G1 | 69.3% | 48.8%* | 50.9%* | 52.2%* | 47.1%* |
| S | 5.8% | 9.8% | 6.7% | 10.2% | 5.4% |
| G2/M | 20.2% | 33.8%** | 35.4%* | 31.1%* | 39.3%** |
Data are given as % of gated cells in G0/G1-, S-, and G2/M-phases. Cells were left untreated (control) or treated with 10 mg/mL of BMO for 72 h. Growth arrest was determined by flow cytometry with the DNA-staining 7-AAD. Results were expressed as means of % gated cells (n = 3). Significant differences to control cells were accepted at
*
P < 0.05 and
**
P < 0.01.