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. 2019 Mar 28;10:238. doi: 10.3389/fphys.2019.00238

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Copyright © 2019 Bellafiore, Battaglia, Bianco and Palma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative microphotographs show the localization of VEGFR-1/Flt-1, VEGFR-2/Flk-1, HIF-1α, and iNOS performed by immunohistochemical analyses in the myocardium of T15 mice. Non-immune mouse serum was substituted for the negative controls. The specificity of the antibodies used was confirmed by the absence of staining in the negative controls.