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. 2019 Mar 28;10:582. doi: 10.3389/fimmu.2019.00582

Figure 6.

Figure 6

PKZ interacts with the mediators of IRF3-dependent and ISGF3-like dependent pathways. (A,B) HEK-293T cells seeded in 10 cm diameter dishes were separately co-transfected PKZ-FLAG with one of the following recombinant plasmids (IRF3-GFP, STING-GFP, ZDHHC1-GFP, eIF2α-GFP, IRF9-GFP, STAT1-GFP, STAT2-GFP, and STAT6-GFP), this experiment was divided into two groups: one group was immunoprecipitated with anti-FLAG antibody and the other group was immunoprecipitated with anti-GFP antibody. Forty eight hours later, cell lysates were separately immunoprecipitated with anti-FLAG (anti-GFP) antibody and IgG. Then the immunoprecipitants were examined by Western blot with the anti-GFP (anti-FLAG) antibody. The molecular masses of IRF3-GFP, STING-GFP, ZDHHC1-GFP, eIF2α-GFP, IRF9-GFP, STAT1-GFP, STAT2-GFP, and STAT6-GFP are 85, 70, 85, 60, 80, 90, 98, and 87 kD, respectively. Each Western blot is representative of at least three independent experiments.