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. 2019 Mar 28;10:567. doi: 10.3389/fimmu.2019.00567

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Copyright © 2019 Cheon, Kim, Choi, Shim, Choi, Jung, Kim, Braciale, Youn, Song and Chang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of IL-17 on vaccine-induced histopathologic alterations in lung structure following RSV challenge. Mice were immunized twice with Gcf via sublingual or intranasal route or with FI-RSV via intramuscular route, and then challenged with RSV A2 strain. Histologic samples were prepared from formalin-fixed, paraffin-embedded lung tissues. (A) H&E staining and (B) PAS staining were performed and examined. (C) PAS-positive cells in peri-bronchial regions were measured for quantitative analysis. Representative results of two independent experiments are shown. All data are expressed in mean ± S.E.M (n = 4). Original magnification for (A) is 10X and the inset shows 40X. Original magnification for (B) is 40X. Scale bars indicate 50 micrometer in 40X. WT, wild type Balb/c mice; KO, IL-17 knock-out mice; ND, Not detected.