Figure 9.

Localization of antigens following sublingual administration. Mice were anesthetized and injected with 5 μg of Alexa Fluor 647-streptavidin conjugate via s.l. or i.n. route. (A) Fluorescence images were measured by IVIS Lumina III with Ex/Em of 640 nm/710 nm at 0.5, 1, and 24 h following injection. Small boxes, draining lymph nodes from sublingual compartment or lung tissue. Average photon radiance of mice treated with the fluorescence dye via (B) s.l. and (C) i.n. route. Representative results of three independent experiments are shown. Avg Radiance, the average photon radiance on the certain surface of a mice was expressed as photons per second per centimeter squared per steradian (p/s/cm²/sr); Gland, Salivary gland; cLN, cervical lymph node; sLN, submandibular lymph node; mdLN, mediastinal lymph node.