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. 2019 Mar 28;10:332. doi: 10.3389/fpls.2019.00332

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Copyright © 2019 Corral-Martínez, Driouich and Seguí-Simarro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Xyloglucan immunogold labeling with the mAb CCRC-M1. (A) Detail of the intine (in) in a pollen-like structure. (B) Detail of the intine and subintinal layer (sl) of an embryogenic microspore. Note that the intine wall is strongly labeled in pollen-like structures (A), but not in embryogenic microspores (B). (C) Inner cell wall (cw) of an embryogenic microspore. (D,E) Torpedo MDEs. Details of the cytoplasm (ct) are shown in (D), where cytoplasmic gold particles strongly decorate the Golgi stacks (gs) and associated vesicles. The cell wall is shown in (E). ex: exine; er: endoplasmic reticulum. Bars: 500 nm.