Table 1.
Primary antibodies (ab) used for immunolocalization in this study.
| Primary ab | Specificity | Provider | Dilution | Secondary ab |
|---|---|---|---|---|
| Callose | ||||
| Anti-callose | Callose | Biosupplies, Australia | 1:5,000 | Goat anti-mouse |
| AGPs | ||||
| JIM8 | AGP | Carbosource, GA, United States | 1:2 | Goat anti-rat |
| JIM14 | AGP | Carbosource, GA, United States | 1:2 | Goat anti-rat |
| JIM16 | AGP | Carbosource, GA, United States | 1:2 | Goat anti-rat |
| JIM13 | AGP | PlantProbes, Leeds, United Kingdom | 1:5 | Goat anti-rat |
| Pectins | ||||
| CCRC-M13 | Rhamnogalacturonan-I | Carbosource, GA, United States | 1:2 | Goat anti-mouse |
| LM5 | (1-4)-β-D-galactan | PlantProbes, Leeds, United Kingdom | undiluted | Goat anti- rat |
| LM6 | (1-5)-α-L-arabinans | PlantProbes, Leeds, United Kingdom | undiluted | Goat anti- rat |
| JIM7 | Highly methyl-esterified homogalacturonans | PlantProbes, Leeds, United Kingdom | 1:2 | Goat anti- rat |
| JIM5 | Low methyl-esterified homogalacturonans | PlantProbes, Leeds, United Kingdom | 1:2 | Goat anti- rat |
| LM7 | Partially methyl-esterified homogalacturonans | PlantProbes, Leeds, United Kingdom | undiluted | Goat anti- rat |
| Hemicelluloses | ||||
| CCRC-M1 | Fucosylated xyloglucan | Carbosource, GA, United States | 1:2 | Goat anti-mouse |
| LM15 | XXXG Xyloglucan | PlantProbes, Leeds, United Kingdom | undiluted | Goat anti- rat |
| LM11 | Arabinoxylan and low-substituted xylan | PlantProbes, Leeds, United Kingdom | undiluted | Goat anti- rat |