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. 2019 Mar 28;7:35. doi: 10.3389/fcell.2019.00035

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Copyright © 2019 Huss, Saias, Hamamah, Singh, Wang, Dave, Kim, Eberwine and Lansford.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Germinal crescent PGCs are actively motile in the presence of fibronectin. Captured frames from live confocal microscope imaging of an HH5 [Tg(hUbC:H2B-Cerulean-2A-Dendra2)] quail embryo that was injected with an antibody against fibronectin (abB3/D6). This antibody was directly conjugated to AlexaFluor 594, red). The 20x images, with 2x optical zoom, are maximum intensity projections of 3 optical slices (2.3 μm each) taken from the dorsal aspect at 1.5 min intervals. Cell nuclei = H2B-Cerulean (blue), cell bodies = Dendra2 (green). While the cells are ubiquitously labeled by the transgene, those with low proliferation rates, such as PGCs (white arrow), are brighter than the surrounding cells. Scale bar = 25 μm. View the full movie in Video S1.