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. 2019 Mar 28;7:35. doi: 10.3389/fcell.2019.00035

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Copyright © 2019 Huss, Saias, Hamamah, Singh, Wang, Dave, Kim, Eberwine and Lansford.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quail PGCs express mRNA for a diverse set of matrix glycoprotein and collagen genes. scRNAseq of select ECM transcripts in PGCs at HH3, HH6, and HH12. (A) Matrix genes along with PGC markers DAZL and DDX4. DAZL = Deleted in azoospermia-like. DDX4 = (Dead-box helices 4 or chicken vasa homolog (CVH). AGRN, Agrin; FN, Fibronectin; LAMA1, Laminin alpha subunit 1; LAMB1, Laminin beta subunit 1; LAMBC1, Laminin gamma subunit 1. (B) Collagen genes. Y axis scaling has been adjusted separately in (A,B). Bars represent the mean + s.e.m. The number of single cells in each group are shown in the legend.