Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 28;7:35. doi: 10.3389/fcell.2019.00035

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Huss, Saias, Hamamah, Singh, Wang, Dave, Kim, Eberwine and Lansford.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

PGCs and their surrounding cells in the germinal crescent co-express transcripts for fibronectin and 2 subunits of laminin. mRNA transcripts for FN1, DAZL, LAMA1, and LAMB1 are expressed by PGCs and their surrounding cells within the germinal crescent of HH4 quail embryos. Wholemount multiplex hybridization chain reaction (HCR) for fibronectin1 (FN1), laminin alpha1 (LAMA1), laminin beta1 (LAMB1), and DAZL mRNA transcripts in HH4-5 quail embryos. (A) HH4. Tiled 10x confocal Z stack maximum intensity projections. From left to right: FN1 (red), DAZL (PGC marker, green), LAMB1 (blue), FN1 and LAMB1 Merge, FN1, DAZL, LAMB1 Merge. Scale bar = 500 μm. (B,C) Images are single optical sections (3.2 um each) from 20x confocal Z stacks with 2x zoom of HH4 (B) and HH5 (C) quail germinal crescent. From left to right: FN1 (red) DAZL (green) LAMB1 or LAMA1 (blue), 3 Color Merge of FN1, DAZL, and LAMB1. Scale bar = 25 μm. (D) Bounding box from B merge, at higher magnification. (E) Bounding box from C merge, at higher magnification. Scale bar = 20 μm.