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. 2019 Mar 28;10:625. doi: 10.3389/fmicb.2019.00625

FIGURE 2.

FIGURE 2

The expression level of miR-17-92 cluster affected EV71 replication. (A) RT-PCR analysis of pri-miR-17-92 cluster expression after HT-29 or Caco-2 cells were transfected with si-pri-mir-17-92 for 48 h. (B, C) HT-29 cells were infected with EV71 (MOI = 1) prior to being transfected with si-pri-mir-17-92 for 24 h. EV71 VP1 expression level was determined via In-cell Western and normalized by DRAQ5 (B), and viral RNA level was quantified via real-time quantitative PCR at indicated time points normalized against GAPDH transcript level (C). (D) Quantitative real-time-PCR analysis of miR-17-92 component expression in HT-29 cells when the cells were transfected with MSCV-miR-17-92 vector or negative control (MSCV-n.c) for 48 h. (E) Titers of progeny EV71 in the culture supernatant of infected-cells before the cells were transfected with MSCV-miR-17-92 vector or negative control. All experiments were performed three times and the representative results were shown. The data were presented as mean ± SEM (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).