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Analysis of long-term humanized mouse model after HTLV-1 infection. Splenocytes from HTLV-1-infected humanized mouse were sorted into CD4+ CD25+ cells and CD4+ CD25− cells by FACS. Genomic DNA isolated from each T-cell population was analyzed for PVL by real-time PCR. PVL is expressed as number of pX copies per 100 cells.
